Sushil Kumar

3 Questions 7 Answers 0 Followers

Questions related from Sushil Kumar

Can someone help me with a problem regarding protein expression in HEK293 cells?

I have cloned five bacterial genes into pFLAG-CMV-6C (Sigma) vector along with two human genes in the same vector. Then I over-expressed these clones into HEK293T cell and lysed the cells after 30...

07 July 2013 3,241 8 View

Does anyone know where I can get a non infectious pseudo HIV-1 entry vector?

I want to check the effect of few HIV inhibitors so I need non-infectious pseudo HIV-1 entry vector. I also read few articles but I could not find it, where can we get this from?

09 September 2012 1,328 2 View

Plasmocytoid dendritic cell (pDC) line or pDC -like cell lines needed.

I need to work on pDC cells for in vitro experimentation. Does anyone have pDC cell lines available? I read few articles in which they wrote we can induce pDC cells like properties in KG-1 cell...

09 September 2012 5,657 3 View