Susan D Kraner

2 Questions 2 Answers 0 Followers

Questions related from Susan D Kraner

What is the best concentration of H2O2 to use for oxidation of recombinant protein?

We have a problem in that storage of our recombinant protein "changes" it and makes it susceptible to binding by an antibody. We think that the recombinant protein may be oxidizing over time, but...

28 October 2024 7,402 5 View

What type of sample prep is needed for SDS-PAGE electrophoresis of plasma?

I mixed EDTA mouse plasma with standard gel sample buffer and got very smeary image on Western with no distinct bands. Is there something in the plasma that needs to be removed before I run gels?

07 December 2023 9,006 1 View