6 Questions 10 Answers 0 Followers
Questions related from Suresh Pujari
Hi all, I am conjugating 15kDa protein with 4.6kbp plasmid but dont know how to confirm the conjugation. is there any way to find out, I dont want to use fluorescence tag or anything. Normal...
03 March 2018 2,864 4 View
I want modify the histone site-specifically. Is there any method known method existing? Thanks in advance.
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I would like to attach Biotin to peptide (10-mer) or protein and then bind it with Streptaavidin. Any suhggestions please?
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Hello, I synthesized 10 mer peptide and want it to incorporate in protein. Could you please suggest me any possible method? Thanks in advance
10 October 2016 1,072 7 View
I synthesized 4-mer peptide and incorporated it into plasmid by reductive amination. But I have to characterize it to make sure the reaction worked. Could anybody please suggest a method to check...
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1) I have to attach fluorescent dye to peptide. But the problem is sensitivity. Which would be the best dye to conjugate with peptide to detect it on 2-5 pmole scale? 2) Apart from fluorescence...
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