Suraj Bhattarai

2 Questions 6 Answers 0 Followers

Questions related from Suraj Bhattarai

How long does 10M ammonium acetate take to dissolve in water?

I need to make 10M ammonium acetate for DNA extraction. I calculated the amount required for 70 ml solution and started dissolving it using a magnetic stirrer. It has been four hours, but the...

03 March 2018 1,631 4 View

What is the minimum reaction volume that can be used in quantitative real time PCR in Stratagene MX 3000 P machine of Agilent Systems?

I have been using 2X SybrGreen mix by Thermofisher Scientific (#F416L) and their recommended volume is 25 ul. I have scaled it down to 15 ul. However, I have read papers where they use 4ul...

09 September 2015 2,088 7 View