9 Questions 25 Answers 0 Followers
Questions related from Sunil Singh
Hi everyone, Can i use Q61L Rac1 as a substitute for the GTP bound state of Rac1; as i came across a paper where they have mentioned that the Q61L Rac1 mimics the GTP bound state of Rac1. Kindly...
04 April 2017 3,682 4 View
Hi everyone, I am using a chemical cross-linker to form a complex of three proteins (A-200kDa; B-21kDa; C- 17kDa) for Cryo EM. I get around 20-30% cross linked complex and the size of this complex...
04 April 2017 5,420 6 View
I am trying to form a complex of proteins using cross linkers. Could you please share the protocol of protein cross-linking using glutaraldehyde? Thanks & Regards, Sunil Singh
09 September 2016 1,194 2 View
Hi everyone, Can Arginine inhibit the crystallization process of a protein? I use 500mM Arginine to suppress the aggregation of my protein (30mM HEPES,pH7.5; 150mM NaCl; 5% Glycerol; 500mM...
07 July 2016 906 6 View
I am trying to form a complex of two proteins, one of which requires Arginine (0.5M) in the GEC buffer for its stability. Has anyone obtained crystals of protein complexes in the presence of...
06 June 2016 6,899 4 View
Hi everyone, I am trying to express and purify a protein in BL21 (DE3) and arctic express RIL cells, which has a membrane binding region. I am using Triton X100 and CHAPS as detergents for its...
05 May 2016 5,122 5 View
what chemicals can be added during purification to inhibit protein protein interaction?
02 February 2016 1,602 15 View
Has anyone expressed and purified proteins with basic PI? I am purifying a protein which has a PI of 8.78. I see a band on SDS-PAGE when protein and solubility tag are together. However, After...
02 February 2016 9,937 8 View
I am expressing a recombinant human protein with MBP solubility tag in E.coli BL21 cells. I am getting a very good expression, However my Gel fiteration profile shows aggregation of cleaved as...
11 November 2015 9,509 8 View
