Sung Won Shin

4 Questions 2 Answers 0 Followers

Questions related from Sung Won Shin

How to effectively differentiate c2c12 myoblasts into myotubes?

I've been working on c2c12 differentiation using this protocol 1. Switch to diff. medium (DMEM, 2% horse serum, 1% penicillin/streptomycin) after reaching full confluence (95~100%) 2. Switch diff....

19 October 2021 8,757 3 View

How to differentiate C2C12 in a single direction / how to achieve unidirectional alignment?

I'm working on myogenic differentiation with c2c12 and I've noticed a lot of figures (c2c12 myotube) searched on Internet have their cells aligned in single direction (without any messy clumps of...

19 October 2021 1,851 4 View

Problem with c2c12 myotube differentiation?

Hi, I'm working on C2C12 myotube differentiation using DMEM (Horse Serum 2% and PEST 1%) and I've noticed that the cell culture looks really dirty. Here's my picture of c2c12 on day 3 of...

03 August 2021 3,951 1 View

Why do SW480 cells lift off or die after PBS washing + sample treatment?

I've been having problems with SW480 cells dying or lifting off after PBS washing or sample treatment. Normally I dispense PBS or sample on the left side of the well, and I noticed that only the...

19 March 2021 8,332 2 View