Sun Hongbin

4 Questions 10 Answers 0 Followers

Questions related from Sun Hongbin

What is the protocal to transfect the CAG repeats plamids in PC12 or SHSY5Y cells?

I used Lipo3000 to transfect the Htt-16Q and Htt-148Q plamids in HEK293 cells and PC12, SHSY5Y cell lines. The results in HEK293 are very good. But, I cant see any Htt expression in transfected...

01 January 2017 2,035 6 View

Should I decrease the conc. and time of ATP in studying the Nlrp3 in BV2 microglia ?

I am studying the Nlrp3 in brain. I chose the BV2 cell lines, and fortunately, I have detected the IL-1beta mature form in the SN after the LPS/ATP(1ug/mL and 2mM, respectively) treatment....

10 October 2016 1,120 0 View

How to dissolve Rotenone ??

Rotenone in Sigma as soluble in DMSO (0.5mg/mL). 1. I wanna 2.5mg/mL concentration to prepare my PD models. How to prepare my solution? 2.If I wanna use Rotenone treat my cells, how to prepare...

08 August 2016 1,838 3 View

Cell lines to research inflammasome ?

I wanna research whether some compounds effect on the inflammasome, especially NLRP3. Considering the raw264.7 donot expreesion ASC, can I use the ASC plasmids-transfected cells (not...

01 January 1970 4,966 7 View