Sumeet Jain

5 Questions 6 Answers 0 Followers

Questions related from Sumeet Jain

Why U937 cells are keep on detaching after PMA treatment ?

Hello everyone, I am treating U937 cells with PMA at 100nM concentration for 24 hr and almost 90% of cells get attached. Then I am culturing these cells with 10% FBS media for the next 24 hr....

01 January 2019 9,690 2 View

Why inhibition in NF-KB reporter cell line is not happening ?

Hello everyone. I have generated DU145- NF-kB reporter cell line (luciferase). In these cells, NF-kB are getting activated with TNF, but not getting inhibited with curcumin or other known NF-kB...

05 May 2016 5,484 4 View

Best method for platelets isolation !

Hello, I am trying isolation of platelets from human and rat blood. I have collected different protocols and one thing is common in all is the use of four different buffer that are ACD, CPD,...

02 February 2016 5,310 4 View

Any input for best method for RNA isolation from fresh and frozen blood ?

I am trying for RNA isolation from fresh and frozen blood with TRIzol and QIAGEN kit but not getting very good RNA. Need suggestions!!!!

01 January 2016 6,898 7 View

Can human PBMCs grow/ survive in RPMI media containing o% or 1% or 5% FBS?

Hello everyone, I want to do some functional study with CM of PBMCs treated with certain drugs. I want to know that what % of FBS will be good?

12 December 2015 4,674 2 View