Suman Samaddar

3 Questions 3 Answers 0 Followers

Questions related from Suman Samaddar

Which sequence to select primers for a two-step RT-qPCR - mRNA sequence or cDNA sequence?

To perform a two-step RT-qPCR (starting material is RNA, then reversed transcribed to cDNA followed by cDNA amplification), do we have to design the sequence-specific primers for cDNA...

24 January 2023 6,442 3 View

Can qPCR primers designed for One-step PCR be also used in two-step PCR?

If we design a primer set against a target intended for their use in one-step qRT-PCR (reverse transcription followed by cDNA to DNA amplification in the same reaction tube), can that same primer...

20 January 2023 6,191 3 View

For normalization of western bands of phospho proteins what should be the loading control?

For normalization of western bands the commonly used loading controls are GAPDH, alpha & beta actin, etc. For phospho proteins should we use the same loading controls or the non-phosphorylated...

09 December 2019 2,320 0 View