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Hello there. Am using pET101 to clone my 1.6 kb insert. Since pET101 is a directional cloning, the cloning method is by overhang CACC at 5' and blunt end at 3' end. Is there anyway 2 insert...
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Hi all, can anybody suggest proteases that can be use for removal of C-terminal histidine without leaving any non-native residues. Thanks!
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