Sudharma Banerjee

4 Questions 8 Answers 0 Followers

Questions related from Sudharma Banerjee

A good protocol for detecting Caspase-3 and cleaved Caspase-3?

Could anyone suggest to me a good protocol to detect Caspase-3 and cleaved Caspase-3? Need to know what percentage of gel to use? Running and transferring conditions?

16 February 2022 4,941 3 View

How much lysis Buffer needed for cell lysis?

Hi, I have an animal cell pellet in a 2 ml Eppendorf tube. Pellet is occupying 40 micro lit space in the tube (If I add 40 miL water in a tube, how much place the water is occupying the same...

31 October 2020 3,453 2 View

Primary Antibody degraded after first use while using Li-cor blocking buffer?

We used to use 5% milk to block NC membrane and incubate the membrane in Primary Antibody (1% Milk and TBST) and we used to reuse the Primary Ab (Storing in -20C). That time it worked nice. Now,...

30 October 2019 8,733 6 View

Will the protein's phosphorylation state could be affected if the samples are sitting into the loading buffer for more than a year?

Will the protein's phosphorylation state could be affected if the samples are sitting into the 4X SDS-PAGE Sample Loading Buffer for more than a year?

26 December 2018 2,610 2 View