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Questions related from Sudharma Banerjee
Could anyone suggest to me a good protocol to detect Caspase-3 and cleaved Caspase-3? Need to know what percentage of gel to use? Running and transferring conditions?
16 February 2022 4,953 3 View
Hi, I have an animal cell pellet in a 2 ml Eppendorf tube. Pellet is occupying 40 micro lit space in the tube (If I add 40 miL water in a tube, how much place the water is occupying the same...
31 October 2020 3,459 2 View
We used to use 5% milk to block NC membrane and incubate the membrane in Primary Antibody (1% Milk and TBST) and we used to reuse the Primary Ab (Storing in -20C). That time it worked nice. Now,...
30 October 2019 8,740 6 View
My MnCl2 solution has been in Room Temperature for more than two years. The color of the solution is clear. Is this solution still stable? Is this good to use for PhosTag experiment?
12 January 2019 3,018 0 View
Will the protein's phosphorylation state could be affected if the samples are sitting into the 4X SDS-PAGE Sample Loading Buffer for more than a year?
26 December 2018 2,617 2 View