Sudharma Banerjee

6 Questions 8 Answers 0 Followers

Questions related from Sudharma Banerjee

A good protocol for detecting Caspase-3 and cleaved Caspase-3?

Could anyone suggest to me a good protocol to detect Caspase-3 and cleaved Caspase-3? Need to know what percentage of gel to use? Running and transferring conditions?

16 February 2022 4,986 3 View

How much lysis Buffer needed for cell lysis?

Hi, I have an animal cell pellet in a 2 ml Eppendorf tube. Pellet is occupying 40 micro lit space in the tube (If I add 40 miL water in a tube, how much place the water is occupying the same...

31 October 2020 3,490 2 View

For Phos-tag: Implication of using ZnCl2 or Zinc Nitrate in sample?

I am thankful to Péter Gyula for providing a valuable suggestion that worked like a miracle in my last experiment. This time my question is I have used Boston Biproducts's RIPA Buffer (BP-115)...

20 November 2019 6,559 0 View

Primary Antibody degraded after first use while using Li-cor blocking buffer?

We used to use 5% milk to block NC membrane and incubate the membrane in Primary Antibody (1% Milk and TBST) and we used to reuse the Primary Ab (Storing in -20C). That time it worked nice. Now,...

30 October 2019 8,776 6 View

Stability of MnCl2 solution?

My MnCl2 solution has been in Room Temperature for more than two years. The color of the solution is clear. Is this solution still stable? Is this good to use for PhosTag experiment?

12 January 2019 3,050 0 View

Will the protein's phosphorylation state could be affected if the samples are sitting into the loading buffer for more than a year?

Will the protein's phosphorylation state could be affected if the samples are sitting into the 4X SDS-PAGE Sample Loading Buffer for more than a year?

26 December 2018 2,665 2 View