Sudhanshu Dwivedi

2 Questions 2 Answers 0 Followers

Questions related from Sudhanshu Dwivedi

What would be the best house keeping gene to study the secondary metabolism related mRNA expression in plant tissues (root and leaf)?

I am working on saponin biosynthesis in medicinal plants, and need to measure the expression of the gene to identify where in plant tissue the gene is exactly expressing.

11 November 2014 276 4 View

How can I clone a gene and pet vector for heterologous expression?

I am trying to clone gene of my interest along with tag to pET24 d (+) vector. As the insert has its own ATG, at the start and another ATG in other frame which is showing restriction site for No...

19 September 2014 5,511 5 View