Subrat Thanapati

4 Questions 12 Answers 0 Followers

Questions related from Subrat Thanapati

What could be the reason for higher percentage of lymphocytes with lower median fluorescence intensity?

I am staining whole blood with antibodies like CD3, CD56 and NKp30 to assesses the expression of NKp30 on NK cells. I am getting increased percentage of NKp30 on NK cells in one group than in...

10 October 2015 1,333 12 View

Is it expected to get high perforin with normal degranulation on NK cell along with high cytotoxicity?

I am working with NK cell cytotoxicity. We found high perforin on NK cells along with cytotoxicity but a normal degranulation in antigen treated PBMCs compared with mock. Any suggestion regarding...

07 July 2015 6,622 2 View

Faint spots in Elispot. What is the reason for this?

Please suggest. I am getting faint spots in elispot, although the spots are visible in naked eye but the Elispot reader can't read it. I am using MSIPS4510 plate (Millipore), also performing the...

08 August 2014 2,208 15 View

Does anybody do NK cell degranulation assay after incubating the whole blood with antigens for 24hrs?

As we know degranulation assay of NK cells is to check the NK cell functionality, so is there anyone doing NK degranulation assay after incubating the whole blood with antigens for 24hrs, then in...

08 August 2013 3,227 2 View