Subhoja Chakraborty

5 Questions 8 Answers 0 Followers

Questions related from Subhoja Chakraborty

How to do ADMET profiling for macromolecular protein inhibitors?

Could anyone suggest me any website or server for evaluating ADMET profiles for protein inhibitors or at the least, for peptides around 11 kDa MW?

27 September 2021 4,474 4 View

Can Cysteine/cystine redox couple be used in place of GSH/GSSG in protein refolding?

I have been recombinantly expressing my protein of interest in pRSET-A in BL21DE3 cells and been refolding using GSSH/GSSG. I would like to know if using a cysteine/cystine redox couple in place...

16 January 2020 1,640 4 View

How do I design colony PCR primers?

I have been attempting Site Directed Mutagenesis on my gene of interest cloned in the vector pET28A+. I began with two mutations, did PCR and transformed the PCR products into BL21DE3 cells. After...

13 August 2018 3,174 6 View

What is the purpose of Sucrose (~say 20%) in a cell pellet wash buffer post cell lysis?

I have been using 2% Triton X-100 followed by 20% sucrose in my cell wash buffer post cell lysis. Triton being a mild detergent, I would like to know the use of sucrose apart from its role in...

17 November 2017 4,159 3 View

Can anybody provide any information on the availability of the fluorescent probe N-(7-Dimethylamino-4-methylcoumarin-3-yl)iodoacetamide/DACIA?

I would also like to know if DACIA is comparable in activity to another thiol protease probe N-(4-(7 - Diethylamino - methylcoumarin - 3 yl)phenyl)maleimide (CPM). Any suggestion will be of great...

16 October 2017 3,038 0 View