Subhashree Ganesan

2 Questions 1 Answers 0 Followers

Questions related from Subhashree Ganesan

Can gel purification kit be converted be used for PCR purification purposes?

I have QIAquick Gel Extraction Kit available with me! Can I use them for PCR clean up with direct PCR product instead of loading onto the gel? This is because my DNA bands do not have any...

28 July 2025 7,454 1 View

Why does my negative control show ct values but no melt curve peaks in RT-PCR?

I am trying to perform relative quantification of telomere length using RT-PCR, with triplicates for each sample, including positive and negative controls. The results showed good Ct values for...

15 May 2025 3,476 0 View