Subhash J Jakhesara

3 Questions 3 Answers 0 Followers

Questions related from Subhash J Jakhesara

Is it necessary to construct viral BAC clone if we want to transfer a gene segment from other virus to it and to generate recombinant virus?

I am working on development of recombinant vaccine and for that I have to insert gene of particular virus in to virus of HVT. Is it must to construct HVT-BAC first or we can go directly for...

07 July 2013 5,247 1 View

Detection of novel splicing events

How to analyze RNA-seq mapping files (BAM files) for detection of all novel splicing events with type of change i.e. 5' or 3' alternative splice site. What I mean is that is there any tool or...

11 November 2012 3,299 4 View

Detection of gene fusions in NGS transcriptome data?

I have lots of sequencing data for a particular cancer I am studying, and I am bit confused about detection of gene fusions. Please can anyone suggest what are the methods and tools available and...

10 October 2012 4,991 1 View