Stuart M Cain

4 Questions 12 Answers 0 Followers

Questions related from Stuart M Cain

Can I use a paired t-test for experimental data acquired on same day?

Hi, hoping to get some help with whether I can use a paired t-test for this dataset. An experiment was run under identical conditions, each day, for nine days. On each day cells in a plate were...

09 May 2023 3,855 4 View

Patch clamping T-lymphocytes: rupturing seal issues?

I'm attempting to patch clamp T-lymphocytes as part of a collaboration. I've been patch clamping for 10+ years in brain slices and cultured neurons / expression systems, but have run into...

13 March 2014 1,700 6 View

Can anyone help with the equipment for transporting brain slices or a section of oxygenated brain tissue?

I need to transport "live" human hippocampal brain tissue for use in electrophysiology and 2P imaging experiments. This is taken from a surgical suite in the hospital to our laboratory, which is...

24 February 2014 4,173 10 View

Can you recommend software for an automated detection of spike-and-wave discharges on EEG traces?

I've been undertaking EEG recordings in the GAERS model of absence seizures which display typical spike-and-wave discharges @ ~6-9Hz interspike frequency. My recordings typically last 30-60 mins...

24 June 2013 9,123 5 View