Stephen G Walker

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Questions related from Stephen G Walker

QPCR standards using 16S primers?

I am using species-specific PCR primers targeted to the 16S rDNA gene. I am cloning the amplified fragment into a plasmid to be used to make the standard curve in quantitative PCR. Should I clone...

21 August 2020 6,964 3 View

When making MS-MUTV medium (selective for S mutans) do you add Chapman Tellurite Sol'n to the cooled autoclaved Mitis Salivarius agar?

Takada K, Hirasawa M: A novelselective medium for isolation of Streptococcus mutans. Journal of Microbiological Methods 60:189-193, 2005. Chapmans Tellurite Solution is 1 ml per L of 1% Potassium...

15 September 2017 2,822 2 View