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Questions related from Stephanie S Coster
I'm looking to automate DNA extractions and I'm trying to figure out which option is better: getting a QIACube to use the DNEasy kits (which seem to have the highest eDNA extraction success of all...
24 May 2021 4,661 3 View
I'm running eDNA samples that have super low DNA concentrations. When I prepare them for qPCR I would like to spin them down, and I have a small benchtop plate spinner centrifuge, but I'm not sure...
16 October 2020 6,202 2 View
I’m using Longmires buffer to store skin swabs. I’m ready for extraction using Qiagens DNeasy kit but wonder if I should use only the swab in the lysis step? I wouldn’t like to discard the buffer...
05 October 2020 4,109 0 View
I see in the Qiagen handbook that it recommends you buy their proprietary centrifuge in order to get high enough rpms (3000 & 6000) necessary for optimal DNA extractions. I have access to a...
12 July 2017 6,432 2 View
An initial test of a methylation sensitive restriction digest did not work (large band rather than smear). The DNA used is precious and I would like to retrieve it (~120 ng). What would the best...
12 July 2017 7,453 4 View
I'm starting a new lab on a small budget. I need to buy a freezer for long term DNA storage. I know that I need a "manual" defrost freezer (rather than a frost-free freezer) to keep the...
24 August 2016 1,941 9 View
When using a population-based genetic distance metric like Fst to test isolation by distance, Fst is often linearized. I'm wondering if one should do the same for individual-based distance metrics?
16 February 2016 3,446 0 View
