Stanislav Kozlovsky

3 Questions 5 Answers 0 Followers

Questions related from Stanislav Kozlovsky

Random hexamer primers in reverse transcription: why don't they block revertase movement?

I'm very embarrassed to admit this, but I don't understand how random hexamer primers (RHP) work in reverse transcription. I made RT with gene-specific or oligo-dT oligos hundreds of times, the...

02 August 2024 3,207 3 View

Sanger sequencing problems: chromatogram quality inconsistency?

Hello, I have problems with plasmid and PCR fragments sequencing (Sanger sequencing on SeqStudio device). We perform sequencing reaction with BrilliantDye™ Terminator v3.1 with subsequent...

12 December 2022 8,219 3 View

Insect culture media compatible with PEI transfection of HighFive cells?

I gonna try PEI-based transient gene expression in insect cells (High Five), the protocol described in: A simple plasmid-based transient gene expression method usin... Authors successfully used...

25 October 2018 8,978 2 View