Stanislav Kozlovsky

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Questions related from Stanislav Kozlovsky

Random hexamer primers in reverse transcription: why don't they block revertase movement?

I'm very embarrassed to admit this, but I don't understand how random hexamer primers (RHP) work in reverse transcription. I made RT with gene-specific or oligo-dT oligos hundreds of times, the...

02 August 2024 3,213 3 View

Sanger sequencing problems: chromatogram quality inconsistency?

Hello, I have problems with plasmid and PCR fragments sequencing (Sanger sequencing on SeqStudio device). We perform sequencing reaction with BrilliantDye™ Terminator v3.1 with subsequent...

12 December 2022 8,224 3 View

Insect culture media compatible with PEI transfection of HighFive cells?

I gonna try PEI-based transient gene expression in insect cells (High Five), the protocol described in: A simple plasmid-based transient gene expression method usin... Authors successfully used...

25 October 2018 8,990 2 View

Wanted: ANY eukaryotic protein poorly expressed in E.coli

Hi, We are looking for model for heterologous protein expression optimization in E.coli. The Inclusion criteria are the following: 1) Eukaryotic gene 2) Poorly expressed in E.coli (ideally –...

01 January 1970 1,085 0 View