What is the HEPES buffer concentration to be used for MCF 10A cell culture without 5% CO2 supply for 6-8 hours?

Hi, I am doing experiments with MCF 10A cells, which were cultured in DMEM/F12 supplemented with 5% horse serum, 20ng/ml of EGF, 0.5µg/ml of hydrocortisone, 100ng/ml of cholera toxin, 10µg/ml of...

27 February 2020 4,027 0 View