Srujan Singh

6 Questions 23 Answers 0 Followers

Questions related from Srujan Singh

Can RIPA lysis buffer be used to perform DNA assay as well?

I am trying to quantify/observe the expression(trend) of MMPs (matrix metalloproteinases) at different time points (i.e. day 0, 3, 5, 7 etc) from ASCs (Adipose derived stem cells) during...

12 December 2018 4,708 5 View

What's the easiest way to synthesize/obtain polycaprolactone (PCL) with two terminal carboxylic groups (-COOH)?

I am working on polycaprolactone (PCL)-protein conjugation. Usually the PCL which is available in market has a hydroxyl group (-OH) group on one of the polymer chain and a carboxylic group (-COOH)...

11 November 2018 3,351 7 View

How to choose the best settings (fit, exposure) for taking images using a stereoscope or an inverted fluorescence microscope?

I have been trying to take images using a stereoscope (Zeiss Stereo Discovery.V8) which comes with the usual AxioVision Software. When I open the AxioCam MRc5 Live window, I usually use the "Best...

10 October 2018 9,496 0 View

What are the different methods for determination of peptide/ protein concentration immobilized onto a polymer surface?

I am trying to immobilize peptide/proteins onto the surface of PCL (polycaprolactone) by making use of EDC/NHS chemistry. Firstly, I want to confirm whether the conjugation was successful....

08 August 2018 1,315 5 View

What's the basic difference between: Aerogel, Xerogel, Cryogel and Hydrogels?

I wanted to know how to qualify/classify a substance as an aerogel or xerogel etc. Is it solely the way in which it is synthesized or is it the final properties of the substance that is...

10 October 2014 3,050 22 View

What is the formula for measuring conductivity of a small slab of sample (of uniform thickness) using four probe method?

I have some (carbonaceous material) samples which are in the shape of slabs with uniform thickness. What formula should I use to measure it's conductivity if I am using four probe method?

09 September 2014 3,378 8 View