Srividya Vaidyanathan

2 Questions 1 Answers 0 Followers

Questions related from Srividya Vaidyanathan

Media composition for MCF10a for mammosphere assay?

Hi, I am planning to do some mammosphere assay for MCF10a cells. This is what I have tried doing so far - Coat the 6 well with agarose and seed 10,000 cells/well with normal MCF10a media without...

08 July 2016 8,352 0 View

Double peaks in my Real Time PCR. Confused of whether to proceed with the data analysis.

I observe double peaks in RT-PCR for the primers that were validated using gel electrophoresis in other samples. I have used these primers on three different sample sets (about twenty-four samples...

10 February 2013 4,036 3 View