Sriram K.K.

3 Questions 8 Answers 0 Followers

Questions related from Sriram K.K.

Why does DNA aggregate when polystyrene sulfonate is added?

Hi, I am doing some single molecule experiments using T7 phage DNA molecules (40 Kb). I tried to add polystyrene sulfonate [poly(sodium 4-styrenesulfonate)] solution (prepared by adding 50 mg...

07 July 2014 2,118 5 View

Can anyone help with DNA contour length measurements (with varying fluorescence intensity)?

I would like to measure contour lengths of fluorescently labeled DNA molecules stretched on a surface. Problem is the fluorescence intensity is not uniform through the DNA length, meaning some...

04 April 2014 9,227 1 View

Labeling proteins with carboxylate fluospheres using EDC

I am trying to find a working protocol to label some proteins (commercially purchased) with carboxylate modified fluospheres (20 or 40 nm size). I figured out that this can be done using a EDC...

06 June 2013 4,291 2 View