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Questions related from Srilatha Badaboina
When drugs half life is 2-3 days and serum samples were collected after 30 and 90 days of drug administration, Is acid dissociation required for these samples ?
15 July 2016 4,229 1 View
I have Adobe photo shop 7.0. I am trying to join 6 images together as layers and trying to make a multiple image panel. The problem is with the background which is in a grey color. I try to change...
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JC-1 dye is well known for using mitochondrial membrane potential disruption detection during apoptosis. JC-1 entry is from the cell membrane to the cytosol to the mitochondria and forms...
29 November 2012 1,719 14 View
Generally my drug has IC-50 value around 10ug/ml. which concentration should I use for time dependent western experiments? 10ug/ml or 15ug/ml or 20ug/ml or 40 ug/ml? which is best and why?
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I would like to check GSH, SOD activity from cell lysates. Can I use cell lysates stored at -20C prepared by RIPA buffer for western analysis?
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We have partially purified a herbal extract (which contains a mixture of flavonoids) in which we found anticancer properties. Further, we checked intracellular ROS generation doses dependently. I...
21 May 2012 2,711 10 View
I am trying to grow hepatic cell lines like HepG2, HUH-7 and ,Hep-3b for anticancer work. I am growing all in RPMI-1640 + 10%FBS + 1X Ab/AM. HUH-7, and hep-3b are growing very nicely but HepG2...
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I would like to detect cleaved caspases (17,19 kd) so Is 15% SDS PAGE ok for this? running SDS PAGE 25mA for 1hr and western transfer conditions i use 100V for 1hr. and any suggestions during...
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