Sri Charitha

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Questions related from Sri Charitha

Hello everyone! I have been doing Immunoprecipitation since long time and unable to avoid IgG bands?

I am doing Immunoprecipitation experiment with A/G beads for pulling down my protein of interest. Even though playing around with IgG rabbit antibody concentration to the protein lysate, still I...

25 September 2023 985 4 View

Hi everyone! I revived RL95 cells yesterday in DMEM F12 medium fresh vial from ATCC. The cells have not attched?

Can anyone who are culturing RL95-2 cells help me in this? The cells look very patchy, aggregated and even after 24 hrs very few have attached.

16 September 2023 8,930 1 View