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Questions related from Sreenivasa Bp
Neutralizing titer is defined as the reciprocal of highest serum dilution required for neutralization of 100 TCID50 of the virus in 50% of the wells. In general, serum is diluted in a twofold...
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Dear fellow researchers, We routinely concentrate BEI inactivated FMDV in TFF. On sucrose density gradient ultracentrifugation, we have observed considerable loss of virus as compared to virus...
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Dear Researchers, Recently we did an experiment. We injected X amount of 'A', 'B' and 'C, viral antigens separately to different groups of cattle. All the three antigens elicited...
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Can anyone share their experience of BEI inactivation of viruses, especially FMDV? How to reduce loss of virus during inactivation? Loss is almost 60% at 1mM final concn of BEI, 37C, 20h, pH8.0....
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Hello, I am learning to use Clone Manager 9.0 for simulation of cloning. I need your help to understand how to simulate cloning of a blunt end PCR product into pCR-Blunt II-TOPO vector. I am...
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Hello everybody, can someone direct me to a tested protocol to get rid off cell culture contaminants which are resistant to antibiotics (Peinicillin, Streptomycin and Kanamycin). It is importnat...
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