What is the best way to isolate total RNA (especially the small RNA less than about 200 bp) from yeast, for RNA-Seq?

I'm looking to isolate the total RNAs from yeast and then prepare them for RNA-Seq. I'll be testing the quality of the RNA with a bioanalyzer. But it seems that most options degrade the RNA or are...

26 September 2014 9,779 4 View