Sophie Wallace

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Questions related from Sophie Wallace

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

07 August 2024 9,573 1 View

How do you quantify cells in coronal mouse sections using ImageJ?

Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...

21 December 2023 7,823 0 View

Bubbles/Sparkles in Sections for Device Placement Confirmation?

My lab is experiencing a new issue with several mouse brain tissue sections (for confirming placement, no immunohistochemistry). Each tissue section has white "sparkles" that dissipate over time...

13 November 2023 1,739 0 View