Sonia Shastri

5 Questions 5 Answers 0 Followers

Questions related from Sonia Shastri

How i do combination studies of wnt inhibitors with chemo drugs by MTT assay ?

I want to do combination studies of various wnt inhibitors with chemotherapeutic drugs. I have calculated their IC50 values by MTT assay. Now i am confused how to design combinations. I am...

07 July 2015 1,228 8 View

Is there any effect of incubating colorectal cells lines for long hours with MTT solution ?

According to my protocol i have to incubate my plate for 1 hr after adding MTT solution. but i incubated for 2.45 hours. My results were not good. Some variations in the OD values. My cell density...

05 May 2015 2,128 4 View

Can anyone tell me about the solubility of niclosamide?

i have made niclosamide stock solution (10mM) in dmso. Further i made serial dilutions in opt- mem for my MTT assay . At concentration of 300 micromolar , i saw some yellow clouds of niclosamide...

05 May 2015 4,033 3 View

Can anyone tell me how to measure the levels of ROS and RNS in mouse. Is there any particular assay ?

I have to measure the level of oxidation products such as 4 HNE, 8-Oxo-DG, nitrotyrosine from all stages of colitis to colorectal cancer development in mouse intestines. I am just wondering if...

01 January 1970 9,709 3 View

Is that possible to extract mitochondria from colonic tissue of mouse?

I want to check reactive oxygen species level in mitochondria before and after giving antioxidant drug in mouse model of colitis. Is that possible to check mitochondrial ROS in colonic tissue or...

01 January 1970 9,559 3 View