Sonia Marina

5 Questions 4 Answers 0 Followers

Questions related from Sonia Marina

Backgrounds in WB from Immunoprecipitation?

Hi, i am getting huge backgrounds from Immunoprecipitation samples when I probe with my antibody of interest from western blot. i am using Dynabeads. Can anyone help me troubleshoot. thank you

05 July 2023 3,464 8 View

Fungi contaminantion in Blocking solution?

Hi, I am using PBS, donkey serum and Tween for blocking solution. Unfortunately , the next day there is fungal contamination in the solution. How can I avoid this contamination for ICC

30 March 2023 6,879 1 View

NSC-34cells in 10 cm dish?

Hi, Do anyone let me know in one 10 c/m dish, approximately how much NSC-34 cells can we get after they are confluent. thank you

23 March 2023 2,789 2 View

What is the difference between MiDi, maxi prep for cloning?

Hi, can anyone explain what is the difference between Midi pre, mini and maxi prep.

08 March 2023 4,758 2 View

Primary Motoneuron culture?

Hi, I am culturing primary Motoneuron from mouse spinal cord on Porn and laminin coated coverslips. By day 7 I get about 10% survival of the neurons. The seeding density was 5000 cells. What can I...

21 February 2023 3,553 1 View