Sonia Belkai

3 Questions 5 Answers 0 Followers

Questions related from Sonia Belkai

Do I have to add feeder cells to my primary T cell line for a 48h incubation ?

Hello everyone, I want to do an ELISA assay to detect IFN gamma and IL2 secreted from a primary T cell line. In order to do so, on day 1 I will thaw these T cells and centrifuge them to get...

06 September 2022 543 1 View

How do I increase the yield of human dendritic cells when isolating from PBMCs ?

Hello everyone, So I am trying to isolate dendritic cells from human PBMCs. I start with a buffycoat of blood from which I isolate the mononucleated cells. I then do a selection of CD14+ cells...

23 May 2022 5,894 4 View

How can I store my lysosomal fractions to preserve the proteins ?

hello everyone, I have prepared lysosomal enriched fractions yesterday and I want to do a western blot on these next week. My proteins of interest are LAMP1, TIM23 and Calnexin. Should I let...

13 January 2022 5,259 4 View