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I extracted casein from skimmed milk using acetic acid and tried dissolving the extracted casein powder in D2O for H'NMR but it just won't dissolve that well and NMR results were not good.
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Do the constraints G1 S phase and G2/M gating have to be the same for all samples in a single run when analyzing the cell cycle using propium iodide or can the parameters be changed?
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