Solomon Oguche

2 Questions 1 Answers 0 Followers

Questions related from Solomon Oguche

Has anyone successfully expressed and purified IgE antibodies in E. coli with high yields?

Hi, I'm seeking advice on expressing and purifying IgE antibodies in a prokaryotic expression system, specifically E. coli. I'm interested in hearing about successful strategies for achieving high...

22 December 2024 1,951 1 View

Has anyone been able to refold and purify unstable enzymes like MMP-9 or any other MMPs using Acetohydroxamic acid (AHA)?

If yes, what concentration did you use that and how did you get rid of the AHA for enzyme activity? I tried using 5uM of AHA, got a good yield, proceeded to removing the AHA with gravity desalting...

20 April 2022 6,420 0 View