Has anyone been able to refold and purify unstable enzymes like MMP-9 or any other MMPs using Acetohydroxamic acid (AHA)?

If yes, what concentration did you use that and how did you get rid of the AHA for enzyme activity? I tried using 5uM of AHA, got a good yield, proceeded to removing the AHA with gravity desalting...

20 April 2022 6,419 0 View