Sofya Novikova

4 Questions 3 Answers 0 Followers

Questions related from Sofya Novikova

How to preserve organoid cell viability before flow cytometry?

I am trying to analyze the shift in the organoid cancer stem cell population after treatment with different compounds in a colorectal cancer patient-derived organoid model. We grow our PDOs in...

27 May 2025 7,158 0 View

Marker set for colorectal cancer-associated cancer stem cells?

Dear ResearchGate Community, I am trying to establish a flow-cytometry based 3 color panel to identify cancer stem cells in patient-derived colorectal cancer organoids. So far I've seen a large...

29 January 2025 5,999 0 View

What causes unspecific binding of isotype ctrl Ab to my organoid cells and how to counteract it?

I have attempted to stain patient-derived cancer organoid cells that have been brought into suspension by accutase and trypsin treatment (for a normal split) for surface markers. I wanted to...

07 August 2024 3,635 0 View

Which membrane material is best for a chemotaxis migration assay with PBMCs?

I am trying to scale up a transwell migration assay for PBMCs and I have preliminary data from 96 well plate format, that shows that my cells migrate towards my attractant through a 5 µm...

17 July 2024 5,335 3 View