Sofie S.

2 Questions 3 Answers 0 Followers

Questions related from Sofie S.

How much plasmid DNA can be transfected in dermal melanocyte cells with Lipofectamine 2000?

I added 3 µg plasmid DNA containing the GFP gene to 75 µL optimem and I added 7,5 µL lipofectamine 2000 reagent to 75 µL optimem. I've incubated the plasmid DNA with lipofectamine and optimem at...

24 September 2021 5,250 3 View

How can I increase bacterial growth in LB medium?

I'd like to characterize an unknown bacteria strain which was stored in a glycerol stock at -80 degrees Celcius. I've inoculated the unknown bacteria strain on an LB agar plate. The colonies of...

15 June 2021 7,865 4 View