Sofia Lage Vickers

3 Questions 4 Answers 0 Followers

Questions related from Sofia Lage Vickers

Why would my protocol ask for EDTA-free protease inhibitor tablets when the lysis buffers are made with EDTA?

Hello, I was wondering if someone could help me understand this. The RIME protocol is a combination of ChIP with MS. In order to lyse the cells, they include 3 different lysis buffers that include...

09 October 2018 2,721 3 View

Amplification in reverse transcription negative controls (no RT enzyme). How to get rid of it?

Hi, I've been having some problems with my RTqPCR results. I use columns for RNA extraction and treat with DNase (in the same columns) before doing the RT. Also, when I designed the primers I made...

31 August 2018 8,048 5 View

Could anyone help me with some troubleshooting I'm having with the matrigel invasion assay?

I've been having trouble with this assay. In the control transwell chambers I see that the cells migrate uniformly, but in the ones with matrigel, only the cells in the edges invade (there is a...

01 January 1970 8,321 3 View