Sneha Singh

3 Questions 19 Answers 0 Followers

Questions related from Sneha Singh

Has anyone had any experience in transfecting endothelial cells on cell culture inserts after forming a monolayer?

And normally also, how to increase the efficiency of transduction in endothelial cells as I am getting a very low efficiency. I have used Lipofectin with 80% confluency.

08 August 2014 7,364 11 View

How can I seed cells on a cell culture insert for a MOI infection?

I have to perform permeability assay by virus infection on endothelial cells. For this I need to use MOI 0.1,1, 10. For this purpose, I need to seed cells on the insert and count them before...

07 July 2014 3,727 4 View

Does anybody have any experience with in vitro permeability assay with virus infection?

I have to perform permeability assay on 6.5mm diameter cell culture inserts with MOI 10. The virus titre is 10^6 pfu/ml. The required cell count per insert is around 10^5 cells/ insert. So, the...

07 July 2014 2,533 6 View