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Questions related from Sneha Roy
Hi All, I am trying to study FRET- live cell imaging with CFP-YFP pair targeted for PM and cytosol in HEK 293T cells. I want to know what settings or parameters should we have to monitor FRET. I...
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Hi, I am investigating an effect of a protein on mTORC1 signaling via western blot and probing for PS6K and P4EBP1, the best known mTORC1 substrates, as well as totals S6K-4EBP1, over expressed...
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I am trying to optimize the Enzchek Phosphate Assay kit (Thermo Fischer Scientific) for a reaction volume of 100uL, while the kit has been designed to work in 1mL. I calculated the reagents...
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I weighed 2.5g of PEG 20000 for a total volume of 5ml to make 50% PEG but the white flakes are hard to dissolve. I want to know if any heating or other treatment is required to dissolve PEG...
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My doubt is about using MCF-7 cell line as a model for assessing the toxicity of fibrils. My fibrils are toxic to this cell line and the compound under study is ameliorating the toxicity....
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What should be the maximum amount of DMSO used when taking CD spectra of protein to avoid the shooting up of the HT voltage. I used 0.1mm cuvette and 0.1%DMSO in my solutions and yet the HT was...
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I want to use Silibinin for my study dissolved in DMSO, molar extinction coefficient of which is not available as such. Can anyone help me with the molar extinction coeffienct of Silibinin in DMSO?
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