Sneha Bhowmick

3 Questions 3 Answers 0 Followers

Questions related from Sneha Bhowmick

Use of sodium acetate ??

Why is 3M sodium acetate used in dna precipitation method along with isopropanol, rather than using sodium chloride ??

02 March 2021 7,047 1 View

Is it possible, that phenotypically ESBL negative isolates will show positive for blaTEM gene when pcr is done ????

I screened 300 isolates, out of which i got 80 phenotypically ESBL positive isolates through antibiotic disk method. And when i tried to detect molecularly through PCR, for blaTEM gene, i got...

26 December 2020 3,327 5 View

Will there be any changes in pcr result if one uses same dna with same PCR conditions and same pcr forward and revwerse primer after 10 months??

Actually, I am doing pcr for Kleb.pneumonia gene. I have did pcr of 90 samples initially and all were positive at that time. But now again when i am repeating the pcr for those 9 samples with same...

21 October 2020 4,505 11 View