7 Questions 7 Answers 0 Followers
Questions related from Smith Bortha
Hello Everyone, Im working on T and B cells. I have to do some imaging and want to attach these cells to plates but they are floating. I tried fibronectin too, but still they are floating, please...
17 August 2018 1,808 4 View
Hello Guys, Im doing a flow for the 10 markers. One of the marker is for the dead cells. I have little confusion, do I have to label all the samples for the dead cells or just one for dead cells ?
17 August 2018 5,047 2 View
Hello Guys, I have to i je t my cells in animal. Our animal facility is 1 hrs traveling distance from lab and I have traspkrt 4T1 cells there. How can O make sure most of the cells arw viable?...
13 August 2018 4,602 3 View
Hello, I have never worked with MTT assay before and im having few problems. I check my cells 231 everyday after treatment, I can see apoptosis in those cells, but MTT assay gives different...
15 March 2018 5,198 5 View
I have the product from drug and PEG/Phospholipid conjugation. I want to purify the drug-Phospholipid and Drug-PEG productions. Any tips will be helpful. Thanks,
21 February 2018 2,952 1 View
Hey Guys, Im wokring with HUVEC for the first time and we have only one vial of HUVEC cells. We have lonza EBM-2 medium but i don't know how to prepare complete growth medium for these cells,...
18 January 2018 9,019 4 View
Hey Guys, Im working on the HUVEC cells for the first time. Usually, we grow them using on 0.1%, gelatin coated dish and Bullet Kit medium from Lonza. Can you please give some tips for the...
13 December 2017 4,572 8 View