Slimane Khayi

6 Questions 20 Answers 0 Followers

Questions related from Slimane Khayi

NGS data analysis

In my shotgun sequencing data I have a low coverage for some contigs. How can I explain that?

09 September 2013 284 3 View

PCR from plasmid DNA failed

I have transformed my DH5alpha using pEPR1(7.3kb) shuttle vector with my insert (1.1kb), after selection of the right clones (checked by colony PCR), I did a miniprep flowed by PCR to check too my...

06 June 2013 1,117 21 View

During sub-cloning into a vector, It is recommended to use two different cloning site?

In order to avoid self ligation of the vector with no insert.

06 June 2013 6,640 6 View

What is the optimal ratio insert:vector for cloning into pGEM, with insert size 1.1 kb and 2.9 kb?

...

05 May 2013 2,683 6 View

TA cloning failure with pGEM-T easy vector

Ligation was ok with my inserts 1.1kb and 2.9kb(checked by PCR sp6/t7 on my ligation reaction) -Transformation of DH5alpha strains then plated on LB with amp/IPTG/x-gal ===> low number of...

05 May 2013 4,780 16 View

Conjugative transfer of Plasmid (pAKE604) from Ecoli S17 to Rhodococcus (Gram+) - I need your recommendation.

To introduce the vector pAKE 604 from a donor(S17) to recipient (Rhodococcus) in order to insert a gene (with mutation) by homologous recombination in the genome of recipient.

05 May 2013 7,128 5 View