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Questions related from Slimane Khayi
In my shotgun sequencing data I have a low coverage for some contigs. How can I explain that?
09 September 2013 285 3 View
I have transformed my DH5alpha using pEPR1(7.3kb) shuttle vector with my insert (1.1kb), after selection of the right clones (checked by colony PCR), I did a miniprep flowed by PCR to check too my...
06 June 2013 1,118 21 View
In order to avoid self ligation of the vector with no insert.
06 June 2013 6,641 6 View
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05 May 2013 2,684 6 View
Ligation was ok with my inserts 1.1kb and 2.9kb(checked by PCR sp6/t7 on my ligation reaction) -Transformation of DH5alpha strains then plated on LB with amp/IPTG/x-gal ===> low number of...
05 May 2013 4,781 16 View
To introduce the vector pAKE 604 from a donor(S17) to recipient (Rhodococcus) in order to insert a gene (with mutation) by homologous recombination in the genome of recipient.
05 May 2013 7,130 5 View