Sleman Bsharat

3 Questions 11 Answers 0 Followers

Questions related from Sleman Bsharat

Who do some researchers use hot lysis buffer instead of RIPA lysis buffer?

Why some researchers use Hot lysis buffer instead of RIPA lysis( with sonication ) ? did the Hot lysis can extract more efficiently nuclear and chromatin bounded proteins? Thanks for any respond

31 March 2015 5,262 1 View

Do you have experience in HEK293 transfection in 6 well plates?

Hi all, can I transfer 2ug of DNA into 6-well plate of HEK293, is it toxic since HEK293 is sensitive? I am using PEI reagent (the gene that I transfect isn't toxic to the cells).

10 November 2014 3,229 26 View

Does someone have experience with 9E10(sc-40) Santacruz anti-myc?

Hi all I want to know in which dilution can i use 9E10 (sc-40) Santacruz anti-myc antibody? and in which solution 1% ? skim milk or something else ? Thanks

29 September 2014 1,265 2 View