Sleman Bsharat

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Questions related from Sleman Bsharat

Do you have experience in HEK293 transfection in 6 well plates?

Hi all, can I transfer 2ug of DNA into 6-well plate of HEK293, is it toxic since HEK293 is sensitive? I am using PEI reagent (the gene that I transfect isn't toxic to the cells).

10 November 2014 3,166 26 View

Does someone have experience with 9E10(sc-40) Santacruz anti-myc?

Hi all I want to know in which dilution can i use 9E10 (sc-40) Santacruz anti-myc antibody? and in which solution 1% ? skim milk or something else ? Thanks

29 September 2014 1,066 2 View