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Questions related from Sivananth Murugesan
I have performed LC-MS for an unknown polymer sample from fermentation broth. Acetonitrile:water mobile phase of 50:50 (vol:vol %) was used. MS data analysis was performed for a single peak which...
03 March 2016 9,910 6 View
I have analysed a polymer sample using 13C NMR and got the following peaks at 30.96, 117.96, 119.81 ,128.45,135.43,147.18 PPM for carbon spectra and as per my interpretation I expect 30.96 -...
02 February 2016 4,361 1 View
After production of PHA when the monomer content is not known , how to analyse the purity or compound structure by doing GC-MS or HPLC; During methanolysis as shown in the paper should different...
06 June 2015 1,447 7 View
when I use C18 Reverse phase column , TFA and acetonitrile as mobile phase , gradient elution at a flow rate of 0.5 ml/min , the retention time is around 3.58 mins and I am not sure about the...
06 June 2015 1,185 4 View
When I try extracting the PHA from biomass using chloroform, I follow the procedure as it has been described in a journal paper. I had to centrifuge the biomass and then the pellet obtained was...
01 January 2015 8,312 10 View
When a microaerophilic microbe is used for the production of PHA and glycerol is used as the sole carbon source what would be the risk of contamination in doing an open batch fermentation process,...
12 December 2014 6,605 14 View
With respect to the reference in the paper (Link) I see that the melting point is quite low for the produced PHA as a result of the carbon source being used, I am using glycerol as the carbon...
12 December 2014 2,093 1 View
After separation of PHA from the biomass, it is usually converted to crotonic acid which is measured with respect to standard to find out the quantity and quality of PHA produced. If I am to...
10 October 2014 4,595 1 View
