Sirlei Antunes Morais

3 Questions 12 Answers 0 Followers

Questions related from Sirlei Antunes Morais

Which is the amount of DNA (ng/ul) expected for 1mg of fresh tissue. For example one mosquito sample?

There is some work on the expected amount of DNA per milligram of tissue.

20 August 2016 4,963 7 View

There is DNA really in mosquitoes kept on mothballs or it's degraded? Someone has amplified DNA from old insects preserved on naphthalene?

I amplifly ITS2 DNA from old mosquito samples preserved on naphthalene using antioxidant, but the target sequence does not amplify, only nonspecific fragments, smaller and more stable. This DNA...

17 August 2016 9,284 3 View

What is the best method to extract DNA from preserved mosquitoes on naphthalene (dry) for a long time?

I have succeeded in extracting DNA from mosquitoes kept on silica or freezer, using appropriate kits. However, in the older samples and preserved on naphthalene, the extracted product is...

13 August 2015 8,645 8 View