Sirjan Sapkota

3 Questions 11 Answers 0 Followers

Questions related from Sirjan Sapkota

What might have caused exclusive non-specific backgrounds in FISH?

The protocol for in situ that I use has been giving me good signals without too much non-specific background until now. This time using a different set of same probes while doing exactly the same...

22 July 2014 6,806 2 View

How can I optimize probe preparation by nick translation?

I have been making DNA probes for in situ for over a year. I use 1.5-2 ug of DNA, 4 ul of dNTP mix (1mM dATP, dCTP, dGTP; 0.66 mM dTTP and 0.33 mM labeled-dUTP), 2 ul 10X buffer, H2O and 2 ul...

12 June 2014 3,077 7 View

Why am I getting milkiness during chromosome pretreatment?

After the formaldehyde/MgCl2 washes I washed the slides in 2X SSC and then when I put the slides in 70% ethanol the slides exude a milky cloud. Why would the slides appear milky during chromosome...

11 June 2014 5,421 11 View