Sindhu Naik

3 Questions 6 Answers 0 Followers

Questions related from Sindhu Naik

Neutrophils - Western Blotting?

I’m new to the field of immunology and don’t have any prior experience working with neutrophils. I’ve been isolating neutrophils from whole blood, lysing the cells directly after isolation with...

30 November 2024 3,280 4 View

Any advice on intact genomic DNA Extraction from Zebrafish embryos?

Hello everyone, I'm facing problems with getting an intact genomic DNA from zebrafish embryos. I have tried to use couple of protocols given in ZFIN network website, but do not see one single band...

29 April 2015 3,332 5 View

Antyone familiar with CRISPR/Cas 9 - gRNA scaffold secondary structure?

Hello, Do anybody face issues or have concerns about the gRNA scaffold's secondary structure formation. During in-vitro transcription, there is no heating and cooling step to enable the formation...

07 April 2015 8,787 2 View