Sin-Hong Pan

2 Questions 1 Answers 0 Followers

Questions related from Sin-Hong Pan

How many cell count are enough to extract protein for western blot?

Hi, I'm currently working on extracting protein from iPSC-derived cardiomyocytes for Western blot analysis. For each experiment, I'm using approximately 8 × 10⁵ cells. I’m wondering whether this...

15 July 2025 8,051 3 View

Can I use alcohol lamp to sterilize cryotube, containing cells?

Hi, I've been culturing iPSC cardiomyocyte cell for 2 months. And every time when I thaw cryotube to seed on 6 well, there must be 1 well that contaminate. I'm thinking that maybe 75% alcohol is...

02 June 2025 8,423 3 View