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I would like to use cyclosporin A in isolated mitochondria. I diluted it in ethanol in order to make a 4 mM stock. However, if I dilute it further in my assay buffer to a concentration of 207 uM...
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I am isolating mitochondria from cell lines and then I do an assay to check for mPTP opening. I use a dye sensitive to calcium and check for uptake and release by mitochondria. However, sometimes...
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