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I am titering anti-mouse CD3 FITC, PE, and APC antibodies for flow cytometry. CD3 is a T-cell marker, but is not expressed until later in T cell development, so its expression is not well...
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We use GFP as a fate map marker in chimeric mice. We are working on a protocol for a skin digest to analyze the turnover of leukocytes in the skin. The protocol works beautifully on a non-GFP...
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We currently use LiberaseTM (2.5mg/mL) in a whole skin digest of murine ear skin. We do not need the level of purity that this reagent provides and are looking for a less expensive alternative....
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